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SF3A1 Rekombinanter Antikörper

SF3A1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250768F4

Kat-Nr. : 86170-2-PBS

Synonyme

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

Filter:
  • Western Blot (WB) analysis of various lysates using SF3A1 Recombinant antibody (86170-2-RR)

    WB analysis using 86170-2-RR (same clone as 86170-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using SF3A1 Recombinant antibody (86170-2-RR)

    WB analysis of rat kidney using 86170-2-RR (same clone as 86170-2-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SF3A1 Recombinant antibody (86170-2-RR)

    IF Staining of HeLa using 86170-2-RR (same clone as 86170-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SF3A1 antibody (86170-2-RR, Clone: 250768F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86170-2-PBS bindet in WB, IF/ICC, Indirect ELISA SF3A1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SF3A1 fusion protein Ag8196
Vollständiger Name splicing factor 3a, subunit 1, 120kDa
Berechnetes Molekulargewicht 793 aa, 89 kDa
Beobachtetes Molekulargewicht120 kDa
GenBank-ZugangsnummerBC007684
Gene symbol SF3A1
Gene ID (NCBI) 10291
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )