SF3B1 Rekombinanter Antikörper

SF3B1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

243145C6

Kat-Nr. : 85640-4-PBS

Synonyme

PRP10, SAP 155, SAP155, SF3b155

---

• 

  WB analysis using 85640-4-RR (same clone as 85640-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85640-4-RR (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85640-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02058-3

  Sandwich ELISA standard curve of MP02058-3, Human SF3B1 Recombinant Matched Antibody Pair - PBS only. 85640-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26689. 85640-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

• 

  Sample test of MP02058-3

  The mean SF3B1 concentration was determined to be 32.11 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load and 48.40 ng/mL in HeLa cell extract based on a 1.8 mg/mL extract load.

• 

  Affinity and Kinetic Characterization of 85640-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85640-4-RR against Human SF3B1 were performed. The affinity constant is 6.07 nM.

---

Geprüfte Anwendungen

Produktinformation

85640-4-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test SF3B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SF3B1 fusion protein Ag26689
• Vollständiger Name: splicing factor 3b, subunit 1, 155kDa
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 146 kDa
• GenBank-Zugangsnummer: NM_012433
• Gene symbol: SF3B1
• Gene ID (NCBI): 23451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.