SF3B1 Rekombinanter Antikörper

SF3B1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243145C6

Kat-Nr. : 85640-4-RR

Synonyme

PRP10, SAP 155, SAP155, SF3b155

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  WB analysis using 85640-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85640-4-RR (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85640-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85640-4-RR against Human SF3B1 were performed. The affinity constant is 6.07 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85640-4-RR bindet in WB, ELISA SF3B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SF3B1 fusion protein Ag26689
• Vollständiger Name: splicing factor 3b, subunit 1, 155kDa
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 146 kDa
• GenBank-Zugangsnummer: NM_012433
• Gene symbol: SF3B1
• Gene ID (NCBI): 23451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.