SF3B2 Polyklonaler Antikörper

SF3B2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 10919-1-AP

Synonyme

Spliceosome-associated protein 145, SF3b150, SF3b145, SAP145, SAP 145

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  WB analysis using 10919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10919-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10919-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 10919-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SF3B2 antibody (10919-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

10919-1-AP bindet in WB, IHC, IF/ICC, ELISA SF3B2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF3B2 fusion protein Ag1009
• Vollständiger Name: splicing factor 3b, subunit 2, 145kDa
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC007610
• Gene symbol: SF3B2
• Gene ID (NCBI): 10992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3B (splicing factor 3B) is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B complex contains the spliceosome-associated protein (SAP) 49, 130, 145, and 155 has been demonstrated. And this complex is essential for U2 snRNP to anchor to the pre-mRNA. Splicing factor 3B subunit 2 (SF3B2), known as SAP145, forms complex with SAP49 which plays a role in cell cycle progression. SAP145, with predicated MW of 100 kDa, appears with MW of about 145 kDa which may be due to the post-translational modification.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; CUGBP2 directly interacts with U2 17S snRNP components and promotes U2 snRNA binding to cardiac troponin T pre-mRNA.; Authors - Goo Young-Hwa YH
human	WB	Cancer Gene Ther; Pan-cancer oncogenic properties and therapeutic potential of SF3B4; Authors - Yanmei Shi
human	WB	Front Oncol; Novel Non-Coding Transcript in NR4A3 Locus, LncNR4A3, Regulates RNA Processing Machinery Proteins and NR4A3 Expression.; Authors - Ada Congrains
human	WB	J Exp Clin Cancer Res; CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.; Authors - Dongni Chen