SF3B4 Polyklonaler Antikörper

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• KD/KO Validated

SF3B4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10482-1-AP

Synonyme

SAP 49, SAP49, SF3B4, SF3b49, SF3b50, Splicing factor 3B subunit 4

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Galerie der Validierungsdaten

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  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10482-1-AP

  IP Result of anti-SF3B4 (IP:10482-1-AP, 4ug; Detection:10482-1-AP 1:500) with HeLa cells lysate 1600ug.

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  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10482-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10482-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10482-1-AP (SF3B4 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10482-1-AP bindet in WB, IP, IHC, IF, ELISA SF3B4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF3B4 fusion protein Ag0737
• Vollständiger Name: splicing factor 3b, subunit 4, 49kDa
• Berechnetes Molekulargewicht: 44 aa, 3 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC004273
• Gene symbol: SF3B4
• Gene ID (NCBI): 10262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human,rat	WB	J Mol Cell Cardiol; Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.; Authors - Scott A Hinger
human	WB,IHC	Cell Death Dis; SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.; Authors - Yuchao Diao ; KD Validated
human	WB,IHC	Cell Death Discov; The splicing factor SF3B4 drives proliferation and invasion in cervical cancer by regulating SPAG5; Authors - Yingwei Li ; KD Validated