SFRP2 Monoklonaler Antikörper

SFRP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1F3A1

Rezensionen (1)

Kat-Nr. : 66328-1-Ig

Synonyme

1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1

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  WB analysis using 66328-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of rat brain using 66328-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66328-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66328-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 66328-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 66328-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 66328-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, COLO 320-Zellen, Maushirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

66328-1-Ig bindet in WB, IHC, IF/ICC, ELISA SFRP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SFRP2 fusion protein Ag18840
• Vollständiger Name: secreted frizzled-related protein 2
• Berechnetes Molekulargewicht: 295 aa, 34 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC008666
• Gene symbol: SFRP2
• Gene ID (NCBI): 6423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Publikationen

Species	Application	Title
human	WB	Exp Cell Res; miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin.; Authors - Xin Tao
Human	IF	Front Immunol; Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction; Authors - Junrui Xu
human,mouse	WB,IHC,IF	Nat Commun; Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring; Authors - Juan Cheng ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-30-2023)

This antibody worked well for mouse tissues when used at 1:200 and incubated for overnight.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver