SFRP4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SFRP4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15328-1-AP

Synonyme

FRP 4, FRPHE, sFRP 4, SFRP4

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 15328-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15328-1-AP

  IP Result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hautkrebsgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Produktinformation

15328-1-AP bindet in WB, IP, IHC, IF, ELISA SFRP4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRP4 fusion protein Ag6909
• Vollständiger Name: secreted frizzled-related protein 4
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 41-51 kDa, 55 kDa
• GenBank-Zugangsnummer: BC058911
• Gene symbol: SFRP4
• Gene ID (NCBI): 6424
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.

Publikationen

Species	Application	Title
human	IHC	Nat Commun; Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin.; Authors - Tracy Tabib
human	IHC	Sci Rep; Expression profiling of prostate cancer tissue delineates genes associated with recurrence after prostatectomy.; Authors - Martin Mørck Mortensen
mouse	WB	Cell Physiol Biochem; Differential Patterns of Secreted Frizzled-Related Protein 4 (SFRP4) in Adipocyte Differentiation: Adipose Depot Specificity.; Authors - Hua Guan ; KD Validated
human,mouse	WB,IHC	Gene Ther; Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.; Authors - Rujue Jin ; KD Validated
human	IHC	Sci Rep; SFRP4 gene expression is increased in aggressive prostate cancer.; Authors - Elise Sandsmark
mouse	WB	Endocrinology; SFRP4 Is a Negative Regulator of Ovarian Follicle Development and Female Fertility.; Authors - Gustavo Zamberlam ; KO Validated