SFRP4 Polyklonaler Antikörper

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• KD/KO Validated

SFRP4 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15328-1-PBS

Synonyme

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• 

  WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

  IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 15328-1-AP (same clone as 15328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15328-1-PBS bindet in WB, IHC, IP, Indirect ELISA SFRP4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRP4 fusion protein Ag6909
• Vollständiger Name: secreted frizzled-related protein 4
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 41-51 kDa, 55 kDa
• GenBank-Zugangsnummer: BC058911
• Gene symbol: SFRP4
• Gene ID (NCBI): 6424
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.