SFRS5 Polyklonaler Antikörper

SFRS5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 16237-1-AP

Synonyme

Delayed early protein HRS, HRS, Pre mRNA splicing factor SRP40, SFRS5, SRP40, SRSF5

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• 

  WB analysis of mouse testis using 16237-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

16237-1-AP bindet in WB, IF, ELISA SFRS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRS5 fusion protein Ag8988
• Vollständiger Name: splicing factor, arginine/serine-rich 5
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• GenBank-Zugangsnummer: BC018823
• Gene symbol: SFRS5
• Gene ID (NCBI): 6430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SR proteins own a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. The binding of SR proteins to pre-mRNA appears to be one of the earliest steps in spliceosome assembly, enhancing the binding of U1 snRNP to the 5' splice site to form a commitment complex [PMID:1577277 ]. Alternative splicing has a major role in the regulation of gene expression. SFRS5 belongs to the serine/arginine (SR) protein family of regulators of alternative pre-mRNA splicing [PMID:9244433].

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB	JCI Insight; Splicing factor SRSF6 mediates pleural fibrosis.; Authors - Li-Mei Liang
mouse		J Dent Res; Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4; Authors - R Shemirani
mouse	WB	Cell Rep; Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing; Authors - Xuan Gu
human	IF	Molecules; L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5; Authors - Junhe Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yan (Verified Customer) (04-28-2021)

Signals was observed in the nucleus when I performed immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells