SFTPB Polyklonaler Antikörper

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• KD/KO Validated

SFTPB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 13664-1-AP

Synonyme

SP-B, Pulmonary surfactant-associated proteolipid SPL(Phe), Pulmonary surfactant-associated protein B, 6 kDa protein, 18 kDa pulmonary-surfactant protein

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  WB analysis of A549 using 13664-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13664-1-AP (SFTPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13664-1-AP (SFTPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

13664-1-AP bindet in WB, IHC, IF, ELISA SFTPB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFTPB fusion protein Ag4576
• Vollständiger Name: surfactant protein B
• Berechnetes Molekulargewicht: 381 aa, 42 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC032785
• Gene symbol: SFTPB
• Gene ID (NCBI): 6439
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pulmonary surfactant-associated protein B(SFTPB) also named SFTP3, is a hydrophobic peptide that facilitates the formation and maintenance of a phospholipid-rich film at the alveolar air-liquid interface. SP-B is necessary for postnatal lung function. SFTPB as a risk biomarker for lung cancer. (PMID:36460583).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Exp Hematol Oncol; Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling; Authors - Hao Luo ; KD Validated
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human	WB,IHC,IF	Lab Invest; Liquid Biopsy in Lung Cancer: Nano-Flow Cytometry Detection of Non-Small Cell Lung Cancer in Blood; Authors - Andong Zhang
human	WB	Environ Toxicol; Silica nanoparticle exposure inhibits surfactant protein A and B in A549 cells through ROS-mediated JNK/c-Jun signaling pathway.; Authors - Xiaojing Yang
human	IHC	Heliyon; Therapeutic and prognostic effect of disulfidptosis-related genes in lung adenocarcinoma; Authors - Changshuan Li
mouse	IHC,IF	Int Immunopharmacol; The impact of POSTN on tumor cell behavior and the tumor microenvironment in lung adenocarcinoma; Authors - Dongfeng Sun