SFTPC Polyklonaler Antikörper

SFTPC Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Rezensionen (1)

Kat-Nr. : 10774-1-AP

Synonyme

PSP C, SFTP2, SFTPC, SMDP2, SP C, SP5, SP-C, surfactant protein C

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Galerie der Validierungsdaten

• 

  WB analysis using 10774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10774-1-AP (SFTPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 10774-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10774-1-AP (SFTPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10774-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10774-1-AP (SFTPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 10774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using SFTPC antibody (10774-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 10774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using SFTPC antibody (10774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 21 publications below

Produktinformation

10774-1-AP bindet in WB, IHC, IF, ELISA SFTPC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFTPC fusion protein Ag1159
• Vollständiger Name: surfactant protein C
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC005913
• Gene symbol: SFTPC
• Gene ID (NCBI): 6440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFTPC (surfactant protein C), also named as SFTP2, SP5 and SP-C, is a pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2). Genetic variations in SFTPC are associated with respiratory distress syndrome in premature infants (RDS). Two isoforms of the human protein are produced by alternative splicing. One previous study reported that SFTPC deletion was observed in NSCLC tissues, implying that SFTPC downregulation might be involved in the progression of lung cancer. (PMID: 35081981, PMID: 31379200)

Publikationen

Species	Application	Title
pig	WB,IF	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
mouse	IF	Oncogene; HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps.; Authors - Zhicheng Zeng
mouse	WB,IF	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human,mouse	IHC	J Pathol; Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia.; Authors - Aibin Liu
mouse	WB,IF	iScience; Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model; Authors - Yahui Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (03-29-2022)

ICC on ALIs without antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary alveolar type 2 cells

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