SFXN1 Polyklonaler Antikörper

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• KD/KO Validated

SFXN1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12296-1-PBS

Synonyme

sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

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  WB analysis using 12296-1-AP (same clone as 12296-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12296-1-AP (same clone as 12296-1-PBS)

  WB result of SFXN1 antibody (12296-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 12296-1-AP (same clone as 12296-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12296-1-AP (same clone as 12296-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12296-1-AP (same clone as 12296-1-PBS)

  IP result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12296-1-PBS bindet in WB, IHC, IP, Indirect ELISA SFXN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFXN1 fusion protein Ag2985
• Vollständiger Name: sideroflexin 1
• Berechnetes Molekulargewicht: 35.6 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC020517
• Gene symbol: SFXN1
• Gene ID (NCBI): 94081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.