SGCZ Polyklonaler Antikörper

SGCZ Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21614-1-AP

Synonyme

ZSG1, Zeta-SG, Zeta-sarcoglycan, Zeta sarcoglycan

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  WB analysis using 21614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse heart using 21614-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SGCZ antibody (21614-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21614-1-AP bindet in WB, IF-P, ELISA SGCZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGCZ fusion protein Ag16305
• Vollständiger Name: sarcoglycan zeta
• Berechnetes Molekulargewicht: 265 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC125037
• Gene symbol: SGCZ
• Gene ID (NCBI): 137868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sarcoglycan zeta, also known as SGCZ, ZSG1, is part of the sarcoglycan complex. The sarcoglycan complex is part of the dystrophin-associated glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. SGCZ plays a role in the maintenance of striated muscle membrane stability. SGCZ was also found as a component of the vascular smooth muscle sarcoglycan complex. SGCZ was reduced at the membrane in muscular dystrophy (PMID: 12189167).