SGEF Polyklonaler Antikörper

SGEF Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12493-1-PBS

Synonyme

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  WB analysis of PC-3 using 12493-1-AP (same clone as 12493-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12493-1-AP (same clone as 12493-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12493-1-AP (same clone as 12493-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 12493-1-AP (same clone as 12493-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12493-1-AP (same clone as 12493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12493-1-AP (same clone as 12493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12493-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12493-1-PBS bindet in WB, IHC, Indirect ELISA SGEF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGEF fusion protein Ag3198
• Vollständiger Name: Src homology 3 domain-containing guanine nucleotide exchange factor
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC016628
• Gene symbol: SGEF
• Gene ID (NCBI): 26084
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.