SGK1 Polyklonaler Antikörper

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• KD/KO Validated

SGK1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 28454-1-AP

Synonyme

SGK, Serum/glucocorticoid-regulated kinase 1, Serine/threonine-protein kinase Sgk1, EC:2.7.11.1

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  WB analysis using 28454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28454-1-AP (SGK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SGK1 antibody (28454-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below

Produktinformation

28454-1-AP bindet in WB, IHC, IF/ICC, ELISA SGK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGK1 fusion protein Ag29420
• Vollständiger Name: serum/glucocorticoid regulated kinase 1
• Berechnetes Molekulargewicht: 431 aa, 49 kDa
• Beobachtetes Molekulargewicht: 42-50 kDa
• GenBank-Zugangsnummer: BC001263
• Gene symbol: SGK1
• Gene ID (NCBI): 6446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.

Publikationen

Species	Application	Title
human,mouse	WB	J Biol Chem; SGK1 negatively regulates inflammatory immune responses and protects against alveolar bone loss through modulation of TRAF3 activity.; Authors - Xiao Han ; KD Validated
human	WB	Int J Mol Sci; PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2; Authors - Qiongfeng Chen
human	WB	Front Oncol; Modeling of senescence-related chemoresistance in ovarian cancer using data analysis and patient-derived organoids; Authors - Xintong Cai
mouse	WB	Int J Biol Sci; Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12; Authors - Xianzheng Zhang
human	WB	Cell Death Dis; CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2; Authors - Di Wang
human	WB	Iran J Basic Med Sci; Quercetin is a foe in the heart by targeting the hERG potassium channel; Authors - Zihao Lu