SGLT1 Polyklonaler Antikörper

SGLT1 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30861-1-PBS

Synonyme

SLC5A1, High affinity sodium-glucose cotransporter

---

• 

  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

  IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

30861-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA SGLT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGLT1 fusion protein Ag29338
• Vollständiger Name: solute carrier family 5 (sodium/glucose cotransporter), member 1
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: NM_000343
• Gene symbol: SGLT1
• Gene ID (NCBI): 6523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).