SGLT2 Polyklonaler Antikörper

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• KD/KO Validated

SGLT2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24654-1-PBS

Synonyme

SLC5A2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter

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  WB analysis using 24654-1-AP (same clone as 24654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 24654-1-AP (same clone as 24654-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24654-1-AP (same clone as 24654-1-PBS)

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SGLT2 antibody (24654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24654-1-PBS bindet in WB, IHC, IF-P, ELISA SGLT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGLT2 fusion protein Ag19163
• Vollständiger Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Berechnetes Molekulargewicht: 672 aa, 73 kDa
• Beobachtetes Molekulargewicht: 69-70 kDa
• GenBank-Zugangsnummer: BC131542
• Gene symbol: SGLT2
• Gene ID (NCBI): 6524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.