SGLT2 Polyklonaler Antikörper

SGLT2 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28683-1-PBS

Synonyme

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  WB analysis using 28683-1-AP (same clone as 28683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28683-1-AP (SGLT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IF Staining of rat kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28683-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA SGLT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGLT2 fusion protein Ag30120
• Vollständiger Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Berechnetes Molekulargewicht: 672 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC131542
• Gene symbol: SGLT2
• Gene ID (NCBI): 6524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.