SGLT3/SLC5A4 Polyklonaler Antikörper

SGLT3/SLC5A4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24327-1-AP

Synonyme

Na(+)/glucose cotransporter 3, SAAT1, SGLT3, SGLT3/SLC5A4, SLC5A4, Sodium/glucose cotransporter 3

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  WB analysis of mouse kidney using 24327-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 24327-1-AP

  IP result of anti-SGLT3/SLC5A4 (IP:24327-1-AP, 4ug; Detection:24327-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 24327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50.

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  IHC staining of human kidney using 24327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24327-1-AP bindet in WB, IP, IF, IHC, ELISA SGLT3/SLC5A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGLT3/SLC5A4 fusion protein Ag19391
• Vollständiger Name: solute carrier family 5 (low affinity glucose cotransporter), member 4
• Berechnetes Molekulargewicht: 659 aa, 72 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC153069
• Gene symbol: SGLT3
• Gene ID (NCBI): 6527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC5A4, also known as SGLT3, is a sugar sensor that depolarizes the plasma membrane in response to external glucose (PMID: 12748858, 20421923). SGLT3 mediates the influx of sodium ions into the cell but does not transport sugars, also potently activated by imino sugars such as deoxynojirimycin (PMID: 22766068, 13130073). SGLT3 is a plasma membrane protein, shares 70% identity with SGLT1, and is expressed in the small intestine (cholinergic neurons), skeletal muscle, kidney, uterus, and testis (PMID: 12748858). SGLT3 protein at apparent molecular masses of 72 kDa and 80 kDa in human kidney tissue and HK-2 cells (PMID: 22766068).

Publikationen

Species	Application	Title
mouse	WB,IF	Int J Environ Res Public Health; Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice.; Authors - Caterina Franco
human	WB,IF	Life Sci; Intestinal sodium/glucose cotransporter 3 expression is epithelial and downregulated in obesity.; Authors - Matúš Soták
human	IHC	PLoS One; Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment.; Authors - Emi Ono