SGMS1 Polyklonaler Antikörper

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• KD/KO Validated

SGMS1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19050-1-AP

Synonyme

hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23

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Galerie der Validierungsdaten

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  WB analysis of human heart using 19050-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19050-1-AP (SGMS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19050-1-AP (SGMS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 19050-1-AP

  IP Result of anti-SGMS1 (IP:19050-1-AP, 4ug; Detection:19050-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19050-1-AP (SGMS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19050-1-AP (SGMS1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19050-1-AP (SGMS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19050-1-AP (SGMS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, humanes Herzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

19050-1-AP bindet in WB, IP, IHC, IF, ELISA SGMS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGMS1 fusion protein Ag5246
• Vollständiger Name: sphingomyelin synthase 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 25 kDa, 49 kDa
• GenBank-Zugangsnummer: BC042899
• Gene symbol: SGMS1
• Gene ID (NCBI): 259230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.

Publikationen

Species	Application	Title
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang ; KD Validated
human	IHC	J Clin Endocrinol Metab; Dysregulated sphingolipid metabolism in endometriosis.; Authors - Yie Hou Lee
mouse	WB,IHC	FASEB J; Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway.; Authors - Mariko Komuro
human,mouse	WB,IF	Exp Neurol; Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.; Authors - Mei-Hong Lu ; KD Validated
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
mouse	IF	Drug Des Devel Ther; Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.; Authors - Yi-Yu Qi