SGSM3 Polyklonaler Antikörper

SGSM3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20825-1-AP

Synonyme

MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3

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Galerie der Validierungsdaten

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  WB analysis of fetal human brain using 20825-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20825-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20825-1-AP

  IP Result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:600) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20825-1-AP (SGSM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, L02-Zellen, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

20825-1-AP bindet in WB, IP, IHC, IF, ELISA SGSM3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGSM3 fusion protein Ag14800
• Vollständiger Name: small G protein signaling modulator 3
• Berechnetes Molekulargewicht: 749 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC008078
• Gene symbol: SGSM3
• Gene ID (NCBI): 27352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.; Authors - Ermes Candiello
mouse	WB	Mediators Inflamm; Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling.; Authors - Yuyi Huang
mouse	WB	Cell Rep; Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.; Authors -
mouse	WB	J Cell Biol; TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1; Authors - Shu Hiragi ; KD Validated