SGSM3 Polyklonaler Antikörper

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• KD/KO Validated

SGSM3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 20825-1-AP

Synonyme

MAP, Merlin associated protein, Merlin-associated protein, RabGAPLP, Rab-GTPase-activating protein-like protein

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  WB analysis using 20825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20825-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20825-1-AP

  IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:2000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 20825-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 20825-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGSM3 antibody (20825-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SGSM3 antibody (20825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 20825-1-AP

  Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, L02-Zellen, Maushirngewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

20825-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SGSM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGSM3 fusion protein Ag14800
• Vollständiger Name: small G protein signaling modulator 3
• Berechnetes Molekulargewicht: 749 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC008078
• Gene symbol: SGSM3
• Gene ID (NCBI): 27352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805).

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.; Authors - Ermes Candiello
mouse	WB	Mediators Inflamm; Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling.; Authors - Yuyi Huang
mouse	WB	Cell Rep; Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.; Authors -
mouse	WB	J Cell Biol; TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1; Authors - Shu Hiragi ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Titouan (Verified Customer) (06-12-2024)

This antibody works very well in WB.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB 1:1000
• Cell Tissue Type: HEK293T, HeLa, Jurkat