SGTA Monoklonaler Antikörper

SGTA Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10B11

Kat-Nr. : 60305-1-PBS

Synonyme

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

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  WB analysis using 60305-1-Ig (same clone as 60305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60305-1-Ig (same clone as 60305-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 60305-1-Ig (same clone as 60305-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60305-1-Ig (same clone as 60305-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60305-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SGTA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SGTA fusion protein Ag1475
• Vollständiger Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-37 kDa
• GenBank-Zugangsnummer: BC008885
• Gene symbol: SGTA
• Gene ID (NCBI): 6449
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.