SH2B1 Polyklonaler Antikörper

SH2B1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12226-1-PBS

Synonyme

SH2B, SH2 domain-containing protein 1B, SH2 B, PSM, Pro-rich, PH and SH2 domain-containing signaling mediator

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  WB analysis of HEK-293 using 12226-1-AP (same clone as 12226-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12226-1-AP (same clone as 12226-1-PBS)

  IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12226-1-AP (same clone as 12226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12226-1-AP (SH2B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 12226-1-AP (same clone as 12226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 12226-1-AP (same clone as 12226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12226-1-AP (same clone as 12226-1-PBS)

  Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12226-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SH2B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH2B1 fusion protein Ag2863
• Vollständiger Name: SH2B adaptor protein 1
• Berechnetes Molekulargewicht: 426 aa, 45 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC010704
• Gene symbol: SH2B1
• Gene ID (NCBI): 25970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).