SH2D1B Polyklonaler Antikörper

SH2D1B Polyklonal Antikörper für FC, IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, FC, CoIP,ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11871-1-AP

Synonyme

EAT 2, EAT2, SH2 domain containing 1B, SH2D1B

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 11871-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11871-1-AP (SH2D1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11871-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11871-1-AP (SH2D1B Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 11871-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11871-1-AP (SH2D1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 11871-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug SH2D1B antibody (11871-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11871-1-AP bindet in IHC, IF, FC, CoIP,ELISA SH2D1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH2D1B fusion protein Ag2454
• Vollständiger Name: SH2 domain containing 1B
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC022407
• Gene symbol: SH2D1B
• Gene ID (NCBI): 117157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH2D1B, also named as EAT2, an adaptor expressed in innate immune cells, including natural killer (NK) cells. It plays a role in controlling signal transduction through at least four receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. EAT-2 overexpression would enhance the innate immune responses and also result in more potent Carcinoembryonic antigen-specific adaptive immune responses.

Publikationen

Species	Application	Title
human	FC	Immunity; Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function.; Authors - Heinrich Schlums
human	FC	Immunity; Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals.; Authors - Jaewon Lee
		J Exp Med; ARID5B regulates metabolic programming in human adaptive NK cells.; Authors - Frank Cichocki
		Mol Ther; Anti-NKG2C/IL-15/anti-CD33 Killer Engager Directs Primary and iPSC-derived NKG2C+ NK cells to Specifically Target Myeloid Leukemia.; Authors - Emily Chiu
human		J Immunol; Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.; Authors - Hagberg Niklas N
human	FC,CoIP	J Immunol; T Cells Regulate Peripheral Naive Mature B Cell Survival by Cell-Cell Contact Mediated through SLAMF6 and SAP.; Authors - Lihi Radomir

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