SH2D2A Polyklonaler Antikörper

SH2D2A Polyklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18539-1-PBS

Synonyme

F2771, SCAP, SH2 domain protein 2A, SH2 domain-containing adapter protein, SH2 domain-containing protein 2A

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  WB analysis of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18539-1-AP (same clone as 18539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18539-1-AP (SH2D2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18539-1-AP (same clone as 18539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18539-1-AP (SH2D2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug SH2D2A Polyclonal antibody (18539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18539-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA SH2D2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH2D2A fusion protein Ag13355
• Vollständiger Name: SH2 domain protein 2A
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC012107
• Gene symbol: SH2D2A
• Gene ID (NCBI): 9047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.