SH2D3A Polyklonaler Antikörper

SH2D3A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15641-1-AP

Synonyme

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

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  WB analysis of HeLa using 15641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15641-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15641-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 15641-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15641-1-AP bindet in WB, IHC, IF/ICC, ELISA SH2D3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH2D3A fusion protein Ag8132
• Vollständiger Name: SH2 domain containing 3A
• Berechnetes Molekulargewicht: 576 aa, 63 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC006281
• Gene symbol: SH2D3A
• Gene ID (NCBI): 10045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

Publikationen

Species	Application	Title
Human	WB	Mol Cell; Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.; Authors - Ok Hyun Park