SH2D5 Polyklonaler Antikörper

SH2D5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55320-1-PBS

Synonyme

SH2 domain-containing protein 5, SH2 domain containing 5

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  WB analysis of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 55320-1-AP (same clone as 55320-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55320-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SH2D5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SH2 domain containing 5
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 43-47 kDa
• GenBank-Zugangsnummer: NM_001103160
• Gene symbol: SH2D5
• Gene ID (NCBI): 400745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.