POSH Polyklonaler Antikörper

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• KD/KO Validated

POSH Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14649-1-AP

Synonyme

KIAA1494, Plenty of SH3s, POSH, Protein POSH, RING finger protein 142, RNF142, SH3 multiple domains protein 2, SH3MD2, SH3RF1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14649-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 14649-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14649-1-AP

  IP Result of anti-POSH (IP:14649-1-AP, 4ug; Detection:14649-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, DU 145-Zellen, HEK-293-Zellen, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

14649-1-AP bindet in WB, IP, IHC, IF, ELISA POSH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POSH fusion protein Ag6243
• Vollständiger Name: SH3 domain containing ring finger 1
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 93-100 kDa
• GenBank-Zugangsnummer: BC053671
• Gene symbol: SH3RF1
• Gene ID (NCBI): 57630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

Publikationen

Species	Application	Title
drosophila	IF	J Cell Biol; Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.; Authors - Ryan J H West
mouse	WB	Int J Mol Sci; Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.; Authors - Sunil Goodwani
mouse	IF	Hum Mol Genet; The Pro-Apoptotic JNK Scaffold POSH/SH3RF1 Mediates CHMP2BIntron5-Associated Toxicity in Animal Models of Frontotemporal Dementia.; Authors - Ryan J H West
human,mouse	WB,IF,IP	Expert Opin Ther Targets; POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.; Authors - Xinshi Wang ; KD Validated
human,mouse	WB,IP	Cell Rep; POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.; Authors - Minghui Yao
human	WB	Int J Biol Macromol; Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein; Authors - Xiangmou Qin