SHARPIN Polyklonaler Antikörper

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• KD/KO Validated

SHARPIN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(109)

Rezensionen (1)

Kat-Nr. : 14626-1-AP

Synonyme

SIPL1, Shank-interacting protein-like 1, Shank-associated RH domain-interacting protein, PSEC0216, hSIPL1

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  WB analysis using 14626-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14626-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14626-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14626-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14626-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14626-1-AP

  IP result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.

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  IHC staining of human lymphoma using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of Jurkat using 14626-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SHARPIN antibody (14626-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 99 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 16 publications below
• CoIP: See 3 publications below

Produktinformation

14626-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SHARPIN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHARPIN fusion protein Ag6201
• Vollständiger Name: SHANK-associated RH domain interactor
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC025244
• Gene symbol: SHARPIN
• Gene ID (NCBI): 81858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Nature; OTULIN limits cell death and inflammation by deubiquitinating LUBAC.; Authors - Klaus Heger
mouse	WB,IP	Nature; LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.; Authors - Nieves Peltzer
human	WB,IP	Cell; OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.; Authors - Keusekotten Kirstin K
human	WB	Cell; The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.; Authors - Rune Busk Damgaard
mouse	WB	Cancer Cell; A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.; Authors - Yannick Boege
mouse	WB	Nat Immunol; Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.; Authors - Yang Shuo S

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katharina (Verified Customer) (07-17-2019)

The antibody worked as expected, detecting a nice band at the right size. Recommended

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells /immunoprecipitates