SHC1 Monoklonaler Antikörper

SHC1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5B1

Kat-Nr. : 67194-2-Ig

Synonyme

SHC, 1A5B1, SH2 domain protein C1, SHC transforming protein 1, SHC-transforming protein 1

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  WB analysis using 67194-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 67194-2-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: TT cells, HeLa-Zellen, hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67194-2-Ig bindet in WB, ELISA SHC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SHC1 fusion protein Ag25173
• Vollständiger Name: SHC (Src homology 2 domain containing) transforming protein 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_183001
• Gene symbol: SHC1
• Gene ID (NCBI): 6464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.