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SHC3 Polyklonaler Antikörper

SHC3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12436-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using SHC3 Polyclonal antibody (12436-1-AP)

    WB analysis using 12436-1-AP (same clone as 12436-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using SHC3 Polyclonal antibody (12436-1-AP)

    IP experiment of BxPC-3 using 12436-1-AP (same clone as 12436-1-PBS)

    IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug. This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SHC3 Polyclonal antibody (12436-1-AP)

    IHC staining of mouse pancreas using 12436-1-AP (same clone as 12436-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SHC3 Polyclonal antibody (12436-1-AP)

    IHC staining of mouse pancreas using 12436-1-AP (same clone as 12436-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12436-1-PBS bindet in WB, IHC, IP, Indirect ELISA SHC3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SHC3 fusion protein Ag3115
Vollständiger Name SHC (Src homology 2 domain containing) transforming protein 3
Berechnetes Molekulargewicht 594 aa, 64 kDa
Beobachtetes Molekulargewicht 50-52 kDa
GenBank-ZugangsnummerBC026314
Gene symbol SHC3
Gene ID (NCBI) 53358
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.