SHCBP1 Monoklonaler Antikörper

SHCBP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B2E12

Kat-Nr. : 68647-1-PBS

Synonyme

SHC SH2 domain-binding protein 1, PAL

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  WB analysis using 68647-1-Ig (same clone as 68647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68647-1-Ig (same clone as 68647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68647-1-Ig (same clone as 68647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68647-1-Ig (same clone as 68647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68647-1-Ig (same clone as 68647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68647-1-Ig (same clone as 68647-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SHCBP1 antibody (68647-1-Ig, Clone: 1B2E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68647-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SHCBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SHCBP1 fusion protein Ag17517
• Vollständiger Name: SHC SH2-domain binding protein 1
• Berechnetes Molekulargewicht: 672 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC030699
• Gene symbol: SHCBP1
• Gene ID (NCBI): 79801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.