Shh Rekombinanter Antikörper

Shh Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240057E7

Kat-Nr. : 83280-1-PBS

Synonyme

M100081, Hxl3, Hx, Hhg1, Dsh

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  WB analysis using 83280-1-RR (same clone as 83280-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 83280-1-RR (same clone as 83280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83280-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83280-1-PBS bindet in WB, IF/ICC, Indirect ELISA Shh und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: sonic hedgehog
• Berechnetes Molekulargewicht: 48KD
• Beobachtetes Molekulargewicht: 19-50 kDa
• GenBank-Zugangsnummer: NM_009170.3
• Gene symbol: Shh
• Gene ID (NCBI): 20423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.