SHH Rekombinanter Antikörper

SHH Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250549A9

Kat-Nr. : 86106-1-RR

Synonyme

EC:3.1.-.-, HHG 1, HHG1, HHG-1, HLP3

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  WB analysis of mouse liver using 86106-1-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 86106-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 86106-1-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 86106-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86106-1-RR bindet in WB, ELISA SHH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SHH fusion protein Eg4010
• Vollständiger Name: sonic hedgehog homolog (Drosophila)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_000193
• Gene symbol: SHH
• Gene ID (NCBI): 6469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. It regulates another target, the gli oncogene. The Shh protein is synthesized as a 45-kDa precursor that undergoes an autocatalytic processing event that produces a 19-kDa N-terminal product, responsible for all signaling activities, and a 25-kDa C-terminal fragment (PMID:10753901, PMID: 16282375). SHH can be detected precursor protein as 48-51-kDa polypeptides (PMID: 15292211).