SHMT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SHMT2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(28)

Kat-Nr. : 11099-1-AP

Synonyme

EC:2.1.2.1, SHMT

---

• 

  WB analysis using 11099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11099-1-AP

  WB result of SHMT2 antibody (11099-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHMT2 transfected HepG2 cells.

• 

  WB analysis of mouse kidney using 11099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11099-1-AP (SHMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Zervixkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

11099-1-AP bindet in WB, IHC, IF/ICC, ELISA SHMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHMT2 fusion protein Ag1584
• Vollständiger Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC011911
• Gene symbol: SHMT2
• Gene ID (NCBI): 6472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	IHC	EMBO Mol Med; Proteomics and personalized PDX models identify treatment for a progressive malignancy within an actionable timeframe; Authors - Georgina D Barnabas
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB,IHC	Am J Respir Cell Mol Biol; Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.; Authors - Robert B Hamanaka
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated