SHMT2 Monoklonaler Antikörper

SHMT2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A2H9

Kat-Nr. : 67980-1-PBS

Synonyme

3A2H9, EC:2.1.2.1, SHMT

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  WB analysis using 67980-1-Ig (same clone as 67980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67980-1-Ig (same clone as 67980-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT2 antibody (67980-1-Ig, Clone: 3A2H9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67980-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SHMT2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SHMT2 fusion protein Ag1584
• Vollständiger Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC011911
• Gene symbol: SHMT2
• Gene ID (NCBI): 6472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.