SHOX2 Polyklonaler Antikörper

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• KD/KO Validated

SHOX2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16366-1-AP

Synonyme

SHOT, Paired-related homeobox protein SHOT, OGI2X, OG12X, OG12

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  WB analysis using 16366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 16366-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16366-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, PC-12-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

16366-1-AP bindet in WB, IHC, ELISA SHOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHOX2 fusion protein Ag9156
• Vollständiger Name: short stature homeobox 2
• Berechnetes Molekulargewicht: 355 aa, 38 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC008829
• Gene symbol: SHOX2
• Gene ID (NCBI): 6474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Genet; A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas; Authors - Peng Ren ; KD Validated
mouse	WB	Oxid Med Cell Longev; NRF-2/HO-1 Pathway-Mediated SHOX2 Activation Is a Key Switch for Heart Rate Acceleration by Yixin-Fumai Granules; Authors - Heng Zhang ; KD Validated
mouse	WB	Aging (Albany NY); D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism; Authors - Heng Zhang
human	WB	Drug Dev Res; Downregulation of short-stature homeobox protein 2 suppresses gastric cancer cell growth and stemness in vitro and in vivo via inactivating wnt/β-catenin signaling; Authors - Xiangyu Chen