SIAE Polyklonaler Antikörper

SIAE Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14566-1-PBS

Synonyme

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

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  WB analysis using 14566-1-AP (same clone as 14566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14566-1-AP (SIAE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14566-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14566-1-PBS bindet in WB, ELISA SIAE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIAE fusion protein Ag6143
• Vollständiger Name: sialic acid acetylesterase
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 62-70 kDa
• GenBank-Zugangsnummer: BC068450
• Gene symbol: SIAE
• Gene ID (NCBI): 54414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acids as terminal sugars of N- and O-glycosylated macromolecules are decisive for the functions of glycoconjugates both in soluble form and integrated into cell membranes .The two enzymes responsible for acetylation and de-acetylation have been described as sialate O-acetyltransferase (SOAT) and sialate O-acetylesterase (SIAE), respectively. SIAE deacetylates α(2-6)-linked sialic acid on N-linked glycans of BCR, allowing the interaction with CD22, that functions in vivo as an inhibitor of BCR signaling. (PMID: 26022516)