SIAE Monoklonaler Antikörper

SIAE Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5F7

Kat-Nr. : 66522-1-PBS

Synonyme

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

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  WB analysis using 66522-1-Ig (same clone as 66522-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 66522-1-Ig (same clone as 66522-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66522-1-Ig (same clone as 66522-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 66522-1-Ig (same clone as 66522-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66522-1-PBS bindet in WB, IF/ICC, Indirect ELISA SIAE und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIAE fusion protein Ag14624
• Vollständiger Name: sialic acid acetylesterase
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC068450
• Gene symbol: SIAE
• Gene ID (NCBI): 54414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.