SIAH1 Polyklonaler Antikörper

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• KD/KO Validated

SIAH1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13886-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13886-1-AP (same clone as 13886-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 13886-1-AP (same clone as 13886-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW264.7 using 13886-1-AP (same clone as 13886-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 13886-1-AP (same clone as 13886-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 13886-1-AP (same clone as 13886-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13886-1-AP (same clone as 13886-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13886-1-PBS bindet in WB, IHC, Indirect ELISA SIAH1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIAH1 fusion protein Ag4336
• Vollständiger Name: seven in absentia homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC035562
• Gene symbol: SIAH1
• Gene ID (NCBI): 6477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)