SIAH1 Rekombinanter Antikörper

SIAH1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240377B4

Kat-Nr. : 83389-4-PBS

Synonyme

Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1

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  WB analysis using 83389-4-RR (same clone as 83389-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 83389-4-RR (same clone as 83389-4-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83389-4-RR (same clone as 83389-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83389-4-RR (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83389-4-RR (same clone as 83389-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Siah1 Recombinant Antibody (83389-4-RR, Clone:240377B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83389-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83389-4-RR against Human SIAH1 were performed. The affinity constant is 2.39 nM.

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Geprüfte Anwendungen

Produktinformation

83389-4-PBS bindet in WB, IHC, ELISA SIAH1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SIAH1 fusion protein Ag34618
• Vollständiger Name: seven in absentia homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC035562
• Gene symbol: SIAH1
• Gene ID (NCBI): 6477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)