SIAH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SIAH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (1)

Kat-Nr. : 12651-1-AP

Synonyme

5N16, E3 ubiquitin-protein ligase SIAH2, EC:2.3.2.27, hSiah2, RING-type E3 ubiquitin transferase SIAH2

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• 

  WB analysis of mouse brain using 12651-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12651-1-AP (SIAH2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis using 12651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12651-1-AP (SIAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12651-1-AP

  IP result of anti-SIAH2 (IP:12651-1-AP, 4ug; Detection:12651-1-AP 1:500) with MCF-7 cells lysate 760 ug.

• 

  IHC staining of mouse brain using 12651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12651-1-AP (SIAH2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12651-1-AP (SIAH2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 12651-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SIAH2 antibody (12651-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, MDA-MB-231-Zellen, Maushirngewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Produktinformation

12651-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SIAH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIAH2 fusion protein Ag3342
• Vollständiger Name: seven in absentia homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 324 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC013082
• Gene symbol: SIAH2
• Gene ID (NCBI): 6478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian Seven-in-absentia homolog 2 (SIAH2), is a RING finger type ubiquitin ligase with a catalytic RING domain on its N-terminus, followed by two zinc fingers and a C-terminal substrate binding domain. Siah is a mammalian homolog of Sina, a Drosophila protein functioning in eye development. Mammals express two isoforms, Siah1 and 2, and mice exhibit two Siah1 isotypes, 1a and 1b. SIAH2 indirectly regulates the level of HIF-1α by promoting degradation of PHDs by UPS. SIAH2, in turn, is positively regulated by hypoxia-dependent transcriptional and posttranscriptional mechanisms.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo ; KD Validated
human	WB	Nucleic Acids Res; P-TEFb is degraded by Siah1/2 in quiescent cells.; Authors - Fang Huang ; KD Validated
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
human	WB,IP	Int J Biol Sci; Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells; Authors - Xiaofeng Dai
		Cell Death Dis; PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.; Authors - Yingying Zhang
mouse	WB,IF	Int Immunopharmacol; Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments; Authors - Guoyao Ou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patrick (Verified Customer) (11-13-2019)

Couldn't detect endogenous or overexpressed SIAH2

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T