SIDT1 Polyklonaler Antikörper

SIDT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55352-1-AP

Synonyme

B830021E24Rik, SID 1, SID1, SIDT1

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Galerie der Validierungsdaten

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  WB analysis using 55352-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55352-1-AP (SIDT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55352-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55352-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SIDT1 antibody (55352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

55352-1-AP bindet in WB, IHC, IF, ELISA SIDT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SID1 transmembrane family, member 1
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 95-110 kDa
• GenBank-Zugangsnummer: NM_017699
• Gene symbol: SIDT1
• Gene ID (NCBI): 54847
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIDT1, also known as a human orthologue of SID-1, belongs to the SID family which is a highly conserved group of transmembrane channel-like proteins. As a transmembrane channel for intercellular communication, SIDT1 was found to facilitate small interfering RNA (siRNA) or miRNA uptake and also involved in cholesterol transport in human systems. SIDT1 mRNA and protein were mainly detected in the stomach and large intestine (including the cecum, colon and rectum) but not in the small intestine (especially the duodenum). Notably, SIDT1 was mainly located in gastric surface mucous cells (pit cells) but barely expressed in the parietal or chief cells. The SIDT1 gene encodes an 827 amino acid protein with a calculated molecular mass of 94 kDa. (PMID: 31632087, PMID: 32801357, PMID: 22174421)

Publikationen

Species	Application	Title
human	WB,IHC	Onco Targets Ther; Integrated Bioinformatics Data Analysis Reveals Prognostic Significance Of SIDT1 In Triple-Negative Breast Cancer.; Authors - Ya Wang