SIGIRR Polyklonaler Antikörper

SIGIRR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 27828-1-AP

Synonyme

Toll/interleukin-1 receptor 8, Toll/interleukin 1 receptor 8, TIR8, Single immunoglobulin domain-containing IL1R-related protein, Single Ig IL-1R-related molecule

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  WB analysis of mouse lung using 27828-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27828-1-AP (SIGIRR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

27828-1-AP bindet in WB, IHC, IF, ELISA SIGIRR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIGIRR fusion protein Ag25850
• Vollständiger Name: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025953
• Gene symbol: SIGIRR
• Gene ID (NCBI): 59307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIGIRR (also known as TIR8) is a member of the toll-like receptor (TLR)/interleukin 1 receptor (IL-1R) superfamily, with a single immunoglobulin extracellular domain and a TIR (Toll/IL-1R) intracellular domain. SIGIRR functions as a negative regulator for IL-1 and LPS signaling, through its interaction with the TLR4 and IL-1R complex. SIGIRR is an important modulator of intestinal epithelial homeostasis and a key regulator of mucosal immunity, maintaining microbial tolerance of the intestinal epithelial layer (PMID: 17398123). The molecular weight of the glycosylated SIGIRR was found to be in the range 50-80 kDa (PMID: 10346978; 17495864).

Publikationen

Species	Application	Title
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
human	WB	Cell Death Dis; IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway.; Authors - Chang Jia
human,mouse	WB,ELISA	Ital J Pediatr; SIGIRR-caspase-8 signaling mediates endothelial apoptosis in Kawasaki disease; Authors - Zhengwang Wen
human	WB,IF	J Control Release; AAV9-delivery of interleukin-37b gene prevents recurrent herpetic stromal keratitis via the SIGIRR pathway in mice; Authors - Jing Wu