Siglec-7 Polyklonaler Antikörper

Siglec-7 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 13939-1-AP

Synonyme

AIRM 1, AIRM1, CD328, CDw328, D siglec, p75, p75/AIRM1, QA79, QA79 membrane protein, SIGLEC 7, SIGLEC7, Siglec-7

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Galerie der Validierungsdaten

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  WB analysis of U-937 using 13939-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13939-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13939-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13939-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13939-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13939-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  FC experiment of U-937 using 13939-1-AP

  1X10^6 U-937 cells were stained with 0.2ug Siglec-7 antibody (13939-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, A549-Zellen, humanes Lebergewebe, humanes Plazenta-Gewebe, K-562-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13939-1-AP bindet in WB, IHC, IF, FC, ELISA Siglec-7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Siglec-7 fusion protein Ag4220
• Vollständiger Name: sialic acid binding Ig-like lectin 7
• Berechnetes Molekulargewicht: 467 aa, 51 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC028150
• Gene symbol: SIGLEC7
• Gene ID (NCBI): 27036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a 75-kDa type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Oncol Lett; Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer.; Authors - Kensuke Yamada

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate