Siglec-9 Polyklonaler Antikörper

Siglec-9 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13377-1-PBS

Synonyme

SIGLEC9, UNQ668/PRO1302, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9

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  WB analysis of HL-60 using 13377-1-AP (same clone as 13377-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 13377-1-AP (same clone as 13377-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 13377-1-AP (same clone as 13377-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 13377-1-AP (same clone as 13377-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 13377-1-AP (same clone as 13377-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Siglec-9 antibody (13377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13377-1-PBS bindet in WB, IHC, IF-P, ELISA Siglec-9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Siglec-9 fusion protein Ag4201
• Vollständiger Name: sialic acid binding Ig-like lectin 9
• Berechnetes Molekulargewicht: 463 aa, 50 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC035365
• Gene symbol: Siglec-9
• Gene ID (NCBI): 27180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).