SIGMAR1 Polyklonaler Antikörper

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• KD/KO Validated

SIGMAR1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 15168-1-AP

Synonyme

hSigmaR1, OPRS1, SIG 1R, Sigma 1 type opioid receptor, Sigma1 receptor, Sigma1R, SIGMAR1, SR BP, SR BP1, SR31747 binding protein, SRBP

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Galerie der Validierungsdaten

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  WB analysis using 15168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15168-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Maus-Kolongewebe, Mausnierengewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

15168-1-AP bindet in WB, IHC, IF, CoIP, ELISA SIGMAR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIGMAR1 fusion protein Ag7351
• Vollständiger Name: sigma non-opioid intracellular receptor 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC004899
• Gene symbol: SIGMAR1
• Gene ID (NCBI): 10280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 28 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
human	WB	Ann Neurol; Hereditary Spastic Paraplegia-Linked REEP1 Modulates ER-Mitochondria Contacts.; Authors - Youngshin Lim
		Proc Natl Acad Sci U S A; DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1.; Authors - Yi Liu
mouse	WB	Schizophr Bull; Allosteric Modulation of the Sigma-1 Receptor Elicits Antipsychotic-like Effects.; Authors - Jiali Chen
mouse	WB	Cell Mol Life Sci; PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis.; Authors - Jessica Knoell
zebrafish	WB	Int J Mol Sci; Sigma-1 Receptor Is Critical for Mitochondrial Activity and Unfolded Protein Response in Larval Zebrafish.; Authors - Lucie Crouzier ; KD Validated

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