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SII-TFIIS,TCEA1 Rekombinanter Antikörper

SII-TFIIS,TCEA1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243155H8

Kat-Nr. : 85664-4-PBS

Synonyme

TCEA1, GTF2S, SII, SII TFIIS, TCEA

Filter:
  • Western Blot (WB) analysis of various lysates using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    WB analysis using 85664-4-RR (same clone as 85664-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of HeLa using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of A431 using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85664-4-PBS bindet in WB, IF/ICC, Indirect ELISA SII-TFIIS,TCEA1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SII-TFIIS,TCEA1 fusion protein Ag12199
Vollständiger Name transcription elongation factor A (SII), 1
Berechnetes Molekulargewicht 301 aa, 34 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC072460
Gene symbol TCEA1
Gene ID (NCBI) 6917
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.