SII-TFIIS,TCEA1 Rekombinanter Antikörper

SII-TFIIS,TCEA1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243155H8

Kat-Nr. : 85664-4-RR

Synonyme

TCEA1, GTF2S, SII, SII TFIIS, TCEA

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  WB analysis using 85664-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85664-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 85664-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, SGC-7901-Zellen, Sp2/0-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85664-4-RR bindet in WB, IF/ICC, ELISA SII-TFIIS,TCEA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SII-TFIIS,TCEA1 fusion protein Ag12199
• Vollständiger Name: transcription elongation factor A (SII), 1
• Berechnetes Molekulargewicht: 301 aa, 34 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC072460
• Gene symbol: TCEA1
• Gene ID (NCBI): 6917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.