SIK1 Polyklonaler Antikörper

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• KD/KO Validated

SIK1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 51045-1-AP

Synonyme

MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK

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Galerie der Validierungsdaten

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  WB analysis of A549 using 51045-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 51045-1-AP

  WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

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  WB analysis using 51045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 51045-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

51045-1-AP bindet in WB, IHC, IF, ELISA SIK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIK1 fusion protein Ag3913
• Vollständiger Name: salt-inducible kinase 1
• Berechnetes Molekulargewicht: 783 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: BC038504
• Gene symbol: SIK1
• Gene ID (NCBI): 150094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

Publikationen

Species	Application	Title
human	WB, IF	J Neuroinflammation; High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.; Authors - Mengyan Hu
human	WB	J Cell Physiol; ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes; Authors - Xueyi Chen
mouse,rat	IHC,IF	J Cereb Blood Flow Metab; Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.; Authors - Cheng Jian J
human	WB	J Leukoc Biol; SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.; Authors - Maria Stella Lombardi
mouse	WB	Inflamm Bowel Dis; HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway.; Authors - Yong Fu
human	WB,IF	Cell Signal; GINS complex protein Sld5 recruits SIK1 to activate MCM helicase during DNA replication.; Authors - Kiranmai Joshi ; KD Validated