Snf1lk Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Snf1lk Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (2)

Kat-Nr. : 51045-1-AP

Synonyme

Sik1, EC:2.7.11.1, HRT-20, Msk, Myocardial SNF1-like kinase

---

• 

  WB analysis of A549 using 51045-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 51045-1-AP

  WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

• 

  WB analysis using 51045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 51045-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

51045-1-AP bindet in WB, IHC, IF/ICC, ELISA Snf1lk und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Snf1lk fusion protein Ag3913
• Vollständiger Name: salt inducible kinase 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: NM_010831
• Gene symbol: Sik1
• Gene ID (NCBI): 17691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

Publikationen

Species	Application	Title
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
human	WB,IHC	iScience; Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus; Authors - Jie Ning ; KD Validated
human	WB,IF	J Neuroinflammation; High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.; Authors - Mengyan Hu
human	WB	J Cell Physiol; ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes; Authors - Xueyi Chen
rat,mouse	IHC,IF	J Cereb Blood Flow Metab; Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.; Authors - Cheng Jian J
human	WB	J Leukoc Biol; SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.; Authors - Maria Stella Lombardi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

SD (Verified Customer) (07-05-2024)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

Snf1lk Antibody Western Blot validation (1000 dilution) in A549 (Cat no:51045-1-AP)

FH

P (Verified Customer) (05-16-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 500
• Cell Tissue Type: AC16

Snf1lk Antibody Western Blot validation (500 dilution) in AC16 (Cat no:51045-1-AP)